rapid stain cbb kit Search Results


rapid barcoding kit  (Oxford Nanopore)
96
Oxford Nanopore rapid barcoding kit
Schematic representation of mechanistic strategies of <t>barcoding.</t> (A–C) Barcodes can be introduced to a template using adaptors through direct ligation (A) , using RT- or PCR primers at the reverse transcription or PCR amplification step (B) , and using hybridizing molecular inversion probes (C) . (D) Schematic representation of the difference between “barcodes” and “sample indexes”. Barcodes aim to correct sequencing errors. For example, a misreading nucleotide, guanosine (G) can be corrected in final consensus sequences for a pool of Sample 1 (top panel). Sample indexes are used to multiplex different sequencing amplicons generated from different pools of samples (Sample 1, 2, and 3) (bottom panel). Panel (A) is modified based on in and panel (C) is modified based on in .
Rapid Barcoding Kit, supplied by Oxford Nanopore, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ache rapid staining kit  (MBL Life science)
90
MBL Life science ache rapid staining kit
Schematic representation of mechanistic strategies of <t>barcoding.</t> (A–C) Barcodes can be introduced to a template using adaptors through direct ligation (A) , using RT- or PCR primers at the reverse transcription or PCR amplification step (B) , and using hybridizing molecular inversion probes (C) . (D) Schematic representation of the difference between “barcodes” and “sample indexes”. Barcodes aim to correct sequencing errors. For example, a misreading nucleotide, guanosine (G) can be corrected in final consensus sequences for a pool of Sample 1 (top panel). Sample indexes are used to multiplex different sequencing amplicons generated from different pools of samples (Sample 1, 2, and 3) (bottom panel). Panel (A) is modified based on in and panel (C) is modified based on in .
Ache Rapid Staining Kit, supplied by MBL Life science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fd rapid golgi stain tm kit  (FD Neuro Technologies Inc)
90
FD Neuro Technologies Inc fd rapid golgi stain tm kit
Schematic representation of mechanistic strategies of <t>barcoding.</t> (A–C) Barcodes can be introduced to a template using adaptors through direct ligation (A) , using RT- or PCR primers at the reverse transcription or PCR amplification step (B) , and using hybridizing molecular inversion probes (C) . (D) Schematic representation of the difference between “barcodes” and “sample indexes”. Barcodes aim to correct sequencing errors. For example, a misreading nucleotide, guanosine (G) can be corrected in final consensus sequences for a pool of Sample 1 (top panel). Sample indexes are used to multiplex different sequencing amplicons generated from different pools of samples (Sample 1, 2, and 3) (bottom panel). Panel (A) is modified based on in and panel (C) is modified based on in .
Fd Rapid Golgi Stain Tm Kit, supplied by FD Neuro Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rapid golgistaintm kit  (FD NeuroTechnologies)
90
FD NeuroTechnologies rapid golgistaintm kit
Schematic representation of mechanistic strategies of <t>barcoding.</t> (A–C) Barcodes can be introduced to a template using adaptors through direct ligation (A) , using RT- or PCR primers at the reverse transcription or PCR amplification step (B) , and using hybridizing molecular inversion probes (C) . (D) Schematic representation of the difference between “barcodes” and “sample indexes”. Barcodes aim to correct sequencing errors. For example, a misreading nucleotide, guanosine (G) can be corrected in final consensus sequences for a pool of Sample 1 (top panel). Sample indexes are used to multiplex different sequencing amplicons generated from different pools of samples (Sample 1, 2, and 3) (bottom panel). Panel (A) is modified based on in and panel (C) is modified based on in .
Rapid Golgistaintm Kit, supplied by FD NeuroTechnologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fd rapid golgistain kit  (FD NeuroTechnologies)
90
FD NeuroTechnologies fd rapid golgistain kit
Evaluation of the dendrites spines in TT, CT, AβPP+, and WT mice. A) <t>Golgi</t> staining shows similar gross visual morphology of neuron and dendritic branching for TT, CT, AβPP+ and WT mice at 6 months of age. Scale bar = 50 μm. B) Sholl analysis (i.e., method of concentric circles) for pyramidal neurons in the cortex reveals a significant effect of genotype (F(3,61) = 15.84, p < 0.000), distance from the soma (F(4,244) = 535.23, p < 0.0001), and a genotype x distance interaction (F(12,2444) = 1.83, p = 0.044) on dendritic arborization. The number of dendrite branches in the intersections was significantly decreased in TT mice starting at 60 μm from the soma. C) There is no difference in the number of the primary branches. D) Representative images of pyramidal neuron spines in the cortex of CT, TT, AβPP+ and WT mice from individual spines identified and counted under 100X magnification using stereological methods. Scale bar = 10 μm. E, F) A significant effect of genotype was found on spine density in the frontal cortex (E) (F(3,278) = 22.07, p < 0.0001), and hippocampus (F) (F(3,136) = 71.28, p < 0.0001). Spine density is expressed as number of spines per 10 μm dendrite length. Post-hoc testing showed significant decreases spine density in CT, TT, and AβPP+ mice as compared to WT mice (p < 0.01) in the frontal cortex and hippocampus, with TT mice having lower values as compared to CT and AβPP+ mice (p < 0.05) (*p < 0.05 compared to WT; #p < 0.05 compared to CT and AβPP+ mice). G) No effect of genotype was observed on spine density in the thalamus.
Fd Rapid Golgistain Kit, supplied by FD NeuroTechnologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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incucyte cytotox green dye  (Sartorius AG)
99
Sartorius AG incucyte cytotox green dye
Evaluation of the dendrites spines in TT, CT, AβPP+, and WT mice. A) <t>Golgi</t> staining shows similar gross visual morphology of neuron and dendritic branching for TT, CT, AβPP+ and WT mice at 6 months of age. Scale bar = 50 μm. B) Sholl analysis (i.e., method of concentric circles) for pyramidal neurons in the cortex reveals a significant effect of genotype (F(3,61) = 15.84, p < 0.000), distance from the soma (F(4,244) = 535.23, p < 0.0001), and a genotype x distance interaction (F(12,2444) = 1.83, p = 0.044) on dendritic arborization. The number of dendrite branches in the intersections was significantly decreased in TT mice starting at 60 μm from the soma. C) There is no difference in the number of the primary branches. D) Representative images of pyramidal neuron spines in the cortex of CT, TT, AβPP+ and WT mice from individual spines identified and counted under 100X magnification using stereological methods. Scale bar = 10 μm. E, F) A significant effect of genotype was found on spine density in the frontal cortex (E) (F(3,278) = 22.07, p < 0.0001), and hippocampus (F) (F(3,136) = 71.28, p < 0.0001). Spine density is expressed as number of spines per 10 μm dendrite length. Post-hoc testing showed significant decreases spine density in CT, TT, and AβPP+ mice as compared to WT mice (p < 0.01) in the frontal cortex and hippocampus, with TT mice having lower values as compared to CT and AβPP+ mice (p < 0.05) (*p < 0.05 compared to WT; #p < 0.05 compared to CT and AβPP+ mice). G) No effect of genotype was observed on spine density in the thalamus.
Incucyte Cytotox Green Dye, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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e irr221 3 3 diaminobenzidine stain reagent dab super plustm high sensitive  (Elabscience Biotechnology)
96
Elabscience Biotechnology e irr221 3 3 diaminobenzidine stain reagent dab super plustm high sensitive
Evaluation of the dendrites spines in TT, CT, AβPP+, and WT mice. A) <t>Golgi</t> staining shows similar gross visual morphology of neuron and dendritic branching for TT, CT, AβPP+ and WT mice at 6 months of age. Scale bar = 50 μm. B) Sholl analysis (i.e., method of concentric circles) for pyramidal neurons in the cortex reveals a significant effect of genotype (F(3,61) = 15.84, p < 0.000), distance from the soma (F(4,244) = 535.23, p < 0.0001), and a genotype x distance interaction (F(12,2444) = 1.83, p = 0.044) on dendritic arborization. The number of dendrite branches in the intersections was significantly decreased in TT mice starting at 60 μm from the soma. C) There is no difference in the number of the primary branches. D) Representative images of pyramidal neuron spines in the cortex of CT, TT, AβPP+ and WT mice from individual spines identified and counted under 100X magnification using stereological methods. Scale bar = 10 μm. E, F) A significant effect of genotype was found on spine density in the frontal cortex (E) (F(3,278) = 22.07, p < 0.0001), and hippocampus (F) (F(3,136) = 71.28, p < 0.0001). Spine density is expressed as number of spines per 10 μm dendrite length. Post-hoc testing showed significant decreases spine density in CT, TT, and AβPP+ mice as compared to WT mice (p < 0.01) in the frontal cortex and hippocampus, with TT mice having lower values as compared to CT and AβPP+ mice (p < 0.05) (*p < 0.05 compared to WT; #p < 0.05 compared to CT and AβPP+ mice). G) No effect of genotype was observed on spine density in the thalamus.
E Irr221 3 3 Diaminobenzidine Stain Reagent Dab Super Plustm High Sensitive, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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golgi–cox staining solution fd rapid golgistain kit  (FD NeuroTechnologies)
90
FD NeuroTechnologies golgi–cox staining solution fd rapid golgistain kit
Evaluation of the dendrites spines in TT, CT, AβPP+, and WT mice. A) <t>Golgi</t> staining shows similar gross visual morphology of neuron and dendritic branching for TT, CT, AβPP+ and WT mice at 6 months of age. Scale bar = 50 μm. B) Sholl analysis (i.e., method of concentric circles) for pyramidal neurons in the cortex reveals a significant effect of genotype (F(3,61) = 15.84, p < 0.000), distance from the soma (F(4,244) = 535.23, p < 0.0001), and a genotype x distance interaction (F(12,2444) = 1.83, p = 0.044) on dendritic arborization. The number of dendrite branches in the intersections was significantly decreased in TT mice starting at 60 μm from the soma. C) There is no difference in the number of the primary branches. D) Representative images of pyramidal neuron spines in the cortex of CT, TT, AβPP+ and WT mice from individual spines identified and counted under 100X magnification using stereological methods. Scale bar = 10 μm. E, F) A significant effect of genotype was found on spine density in the frontal cortex (E) (F(3,278) = 22.07, p < 0.0001), and hippocampus (F) (F(3,136) = 71.28, p < 0.0001). Spine density is expressed as number of spines per 10 μm dendrite length. Post-hoc testing showed significant decreases spine density in CT, TT, and AβPP+ mice as compared to WT mice (p < 0.01) in the frontal cortex and hippocampus, with TT mice having lower values as compared to CT and AβPP+ mice (p < 0.05) (*p < 0.05 compared to WT; #p < 0.05 compared to CT and AβPP+ mice). G) No effect of genotype was observed on spine density in the thalamus.
Golgi–Cox Staining Solution Fd Rapid Golgistain Kit, supplied by FD NeuroTechnologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hemacolor rapid staining kit  (Merck KGaA)
90
Merck KGaA hemacolor rapid staining kit
Evaluation of the dendrites spines in TT, CT, AβPP+, and WT mice. A) <t>Golgi</t> staining shows similar gross visual morphology of neuron and dendritic branching for TT, CT, AβPP+ and WT mice at 6 months of age. Scale bar = 50 μm. B) Sholl analysis (i.e., method of concentric circles) for pyramidal neurons in the cortex reveals a significant effect of genotype (F(3,61) = 15.84, p < 0.000), distance from the soma (F(4,244) = 535.23, p < 0.0001), and a genotype x distance interaction (F(12,2444) = 1.83, p = 0.044) on dendritic arborization. The number of dendrite branches in the intersections was significantly decreased in TT mice starting at 60 μm from the soma. C) There is no difference in the number of the primary branches. D) Representative images of pyramidal neuron spines in the cortex of CT, TT, AβPP+ and WT mice from individual spines identified and counted under 100X magnification using stereological methods. Scale bar = 10 μm. E, F) A significant effect of genotype was found on spine density in the frontal cortex (E) (F(3,278) = 22.07, p < 0.0001), and hippocampus (F) (F(3,136) = 71.28, p < 0.0001). Spine density is expressed as number of spines per 10 μm dendrite length. Post-hoc testing showed significant decreases spine density in CT, TT, and AβPP+ mice as compared to WT mice (p < 0.01) in the frontal cortex and hippocampus, with TT mice having lower values as compared to CT and AβPP+ mice (p < 0.05) (*p < 0.05 compared to WT; #p < 0.05 compared to CT and AβPP+ mice). G) No effect of genotype was observed on spine density in the thalamus.
Hemacolor Rapid Staining Kit, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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developing solution rapid golgi stain kit  (FD NeuroTechnologies)
90
FD NeuroTechnologies developing solution rapid golgi stain kit
Evaluation of the dendrites spines in TT, CT, AβPP+, and WT mice. A) <t>Golgi</t> staining shows similar gross visual morphology of neuron and dendritic branching for TT, CT, AβPP+ and WT mice at 6 months of age. Scale bar = 50 μm. B) Sholl analysis (i.e., method of concentric circles) for pyramidal neurons in the cortex reveals a significant effect of genotype (F(3,61) = 15.84, p < 0.000), distance from the soma (F(4,244) = 535.23, p < 0.0001), and a genotype x distance interaction (F(12,2444) = 1.83, p = 0.044) on dendritic arborization. The number of dendrite branches in the intersections was significantly decreased in TT mice starting at 60 μm from the soma. C) There is no difference in the number of the primary branches. D) Representative images of pyramidal neuron spines in the cortex of CT, TT, AβPP+ and WT mice from individual spines identified and counted under 100X magnification using stereological methods. Scale bar = 10 μm. E, F) A significant effect of genotype was found on spine density in the frontal cortex (E) (F(3,278) = 22.07, p < 0.0001), and hippocampus (F) (F(3,136) = 71.28, p < 0.0001). Spine density is expressed as number of spines per 10 μm dendrite length. Post-hoc testing showed significant decreases spine density in CT, TT, and AβPP+ mice as compared to WT mice (p < 0.01) in the frontal cortex and hippocampus, with TT mice having lower values as compared to CT and AβPP+ mice (p < 0.05) (*p < 0.05 compared to WT; #p < 0.05 compared to CT and AβPP+ mice). G) No effect of genotype was observed on spine density in the thalamus.
Developing Solution Rapid Golgi Stain Kit, supplied by FD NeuroTechnologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fd rapid golgi stain kit  (FD NeuroTechnologies)
90
FD NeuroTechnologies fd rapid golgi stain kit
Evaluation of the dendrites spines in TT, CT, AβPP+, and WT mice. A) <t>Golgi</t> staining shows similar gross visual morphology of neuron and dendritic branching for TT, CT, AβPP+ and WT mice at 6 months of age. Scale bar = 50 μm. B) Sholl analysis (i.e., method of concentric circles) for pyramidal neurons in the cortex reveals a significant effect of genotype (F(3,61) = 15.84, p < 0.000), distance from the soma (F(4,244) = 535.23, p < 0.0001), and a genotype x distance interaction (F(12,2444) = 1.83, p = 0.044) on dendritic arborization. The number of dendrite branches in the intersections was significantly decreased in TT mice starting at 60 μm from the soma. C) There is no difference in the number of the primary branches. D) Representative images of pyramidal neuron spines in the cortex of CT, TT, AβPP+ and WT mice from individual spines identified and counted under 100X magnification using stereological methods. Scale bar = 10 μm. E, F) A significant effect of genotype was found on spine density in the frontal cortex (E) (F(3,278) = 22.07, p < 0.0001), and hippocampus (F) (F(3,136) = 71.28, p < 0.0001). Spine density is expressed as number of spines per 10 μm dendrite length. Post-hoc testing showed significant decreases spine density in CT, TT, and AβPP+ mice as compared to WT mice (p < 0.01) in the frontal cortex and hippocampus, with TT mice having lower values as compared to CT and AβPP+ mice (p < 0.05) (*p < 0.05 compared to WT; #p < 0.05 compared to CT and AβPP+ mice). G) No effect of genotype was observed on spine density in the thalamus.
Fd Rapid Golgi Stain Kit, supplied by FD NeuroTechnologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rapid giemsa staining kit  (Brickell Biotech)
86
Brickell Biotech rapid giemsa staining kit
Evaluation of the dendrites spines in TT, CT, AβPP+, and WT mice. A) <t>Golgi</t> staining shows similar gross visual morphology of neuron and dendritic branching for TT, CT, AβPP+ and WT mice at 6 months of age. Scale bar = 50 μm. B) Sholl analysis (i.e., method of concentric circles) for pyramidal neurons in the cortex reveals a significant effect of genotype (F(3,61) = 15.84, p < 0.000), distance from the soma (F(4,244) = 535.23, p < 0.0001), and a genotype x distance interaction (F(12,2444) = 1.83, p = 0.044) on dendritic arborization. The number of dendrite branches in the intersections was significantly decreased in TT mice starting at 60 μm from the soma. C) There is no difference in the number of the primary branches. D) Representative images of pyramidal neuron spines in the cortex of CT, TT, AβPP+ and WT mice from individual spines identified and counted under 100X magnification using stereological methods. Scale bar = 10 μm. E, F) A significant effect of genotype was found on spine density in the frontal cortex (E) (F(3,278) = 22.07, p < 0.0001), and hippocampus (F) (F(3,136) = 71.28, p < 0.0001). Spine density is expressed as number of spines per 10 μm dendrite length. Post-hoc testing showed significant decreases spine density in CT, TT, and AβPP+ mice as compared to WT mice (p < 0.01) in the frontal cortex and hippocampus, with TT mice having lower values as compared to CT and AβPP+ mice (p < 0.05) (*p < 0.05 compared to WT; #p < 0.05 compared to CT and AβPP+ mice). G) No effect of genotype was observed on spine density in the thalamus.
Rapid Giemsa Staining Kit, supplied by Brickell Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Schematic representation of mechanistic strategies of barcoding. (A–C) Barcodes can be introduced to a template using adaptors through direct ligation (A) , using RT- or PCR primers at the reverse transcription or PCR amplification step (B) , and using hybridizing molecular inversion probes (C) . (D) Schematic representation of the difference between “barcodes” and “sample indexes”. Barcodes aim to correct sequencing errors. For example, a misreading nucleotide, guanosine (G) can be corrected in final consensus sequences for a pool of Sample 1 (top panel). Sample indexes are used to multiplex different sequencing amplicons generated from different pools of samples (Sample 1, 2, and 3) (bottom panel). Panel (A) is modified based on in and panel (C) is modified based on in .

Journal: Frontiers in Molecular Biosciences

Article Title: A systematic review of the barcoding strategy that contributes to COVID-19 diagnostics at a population level

doi: 10.3389/fmolb.2023.1141534

Figure Lengend Snippet: Schematic representation of mechanistic strategies of barcoding. (A–C) Barcodes can be introduced to a template using adaptors through direct ligation (A) , using RT- or PCR primers at the reverse transcription or PCR amplification step (B) , and using hybridizing molecular inversion probes (C) . (D) Schematic representation of the difference between “barcodes” and “sample indexes”. Barcodes aim to correct sequencing errors. For example, a misreading nucleotide, guanosine (G) can be corrected in final consensus sequences for a pool of Sample 1 (top panel). Sample indexes are used to multiplex different sequencing amplicons generated from different pools of samples (Sample 1, 2, and 3) (bottom panel). Panel (A) is modified based on in and panel (C) is modified based on in .

Article Snippet: Primer-associated approach , Sequence-based barcodes , SQK-RBK004: transposase carrying barcodes to the site of the cleavage , - , - , Whole genome , Oxford Nanopore Rapid Barcoding kit (SQK-RBK004) , SARS-CoV-2 patient samples (nasopharyngeal swab) , Oxford Nanopore , Guppy version 3.6.0; ARTIC Network bioinformatics protocol , Multiplex samples , Propose a method to sequence the whole genome of SARS-CoV-2 in a rapid and cost-efficient manner , .

Techniques: Ligation, Reverse Transcription, Amplification, Sequencing, Multiplex Assay, Generated, Modification

Systematic comparison of barcoding strategies used in the category of molecular barcodes.

Journal: Frontiers in Molecular Biosciences

Article Title: A systematic review of the barcoding strategy that contributes to COVID-19 diagnostics at a population level

doi: 10.3389/fmolb.2023.1141534

Figure Lengend Snippet: Systematic comparison of barcoding strategies used in the category of molecular barcodes.

Article Snippet: Primer-associated approach , Sequence-based barcodes , SQK-RBK004: transposase carrying barcodes to the site of the cleavage , - , - , Whole genome , Oxford Nanopore Rapid Barcoding kit (SQK-RBK004) , SARS-CoV-2 patient samples (nasopharyngeal swab) , Oxford Nanopore , Guppy version 3.6.0; ARTIC Network bioinformatics protocol , Multiplex samples , Propose a method to sequence the whole genome of SARS-CoV-2 in a rapid and cost-efficient manner , .

Techniques: Comparison, Software, Sequencing, Multiplex Assay, CRISPR, Plasmid Preparation, Microarray, Binding Assay, Amplification, Extraction, Ligation, DNA Sequencing, Multiplexing, Generated, Reverse Transcription, Staining, Flow Cytometry, High Throughput Screening Assay, Inhibition, Blocking Assay, Conjugation Assay, RNA Sequencing Assay, Transmission Assay, Incubation, Diagnostic Assay, Next-Generation Sequencing, Infection

Evaluation of the dendrites spines in TT, CT, AβPP+, and WT mice. A) Golgi staining shows similar gross visual morphology of neuron and dendritic branching for TT, CT, AβPP+ and WT mice at 6 months of age. Scale bar = 50 μm. B) Sholl analysis (i.e., method of concentric circles) for pyramidal neurons in the cortex reveals a significant effect of genotype (F(3,61) = 15.84, p < 0.000), distance from the soma (F(4,244) = 535.23, p < 0.0001), and a genotype x distance interaction (F(12,2444) = 1.83, p = 0.044) on dendritic arborization. The number of dendrite branches in the intersections was significantly decreased in TT mice starting at 60 μm from the soma. C) There is no difference in the number of the primary branches. D) Representative images of pyramidal neuron spines in the cortex of CT, TT, AβPP+ and WT mice from individual spines identified and counted under 100X magnification using stereological methods. Scale bar = 10 μm. E, F) A significant effect of genotype was found on spine density in the frontal cortex (E) (F(3,278) = 22.07, p < 0.0001), and hippocampus (F) (F(3,136) = 71.28, p < 0.0001). Spine density is expressed as number of spines per 10 μm dendrite length. Post-hoc testing showed significant decreases spine density in CT, TT, and AβPP+ mice as compared to WT mice (p < 0.01) in the frontal cortex and hippocampus, with TT mice having lower values as compared to CT and AβPP+ mice (p < 0.05) (*p < 0.05 compared to WT; #p < 0.05 compared to CT and AβPP+ mice). G) No effect of genotype was observed on spine density in the thalamus.

Journal: Journal of Alzheimer's disease : JAD

Article Title: Corticotrophin Releasing Factor Accelerates Neuropathology and Cognitive Decline in a Mouse Model of Alzheimer's Disease

doi: 10.3233/JAD-2011-111328

Figure Lengend Snippet: Evaluation of the dendrites spines in TT, CT, AβPP+, and WT mice. A) Golgi staining shows similar gross visual morphology of neuron and dendritic branching for TT, CT, AβPP+ and WT mice at 6 months of age. Scale bar = 50 μm. B) Sholl analysis (i.e., method of concentric circles) for pyramidal neurons in the cortex reveals a significant effect of genotype (F(3,61) = 15.84, p < 0.000), distance from the soma (F(4,244) = 535.23, p < 0.0001), and a genotype x distance interaction (F(12,2444) = 1.83, p = 0.044) on dendritic arborization. The number of dendrite branches in the intersections was significantly decreased in TT mice starting at 60 μm from the soma. C) There is no difference in the number of the primary branches. D) Representative images of pyramidal neuron spines in the cortex of CT, TT, AβPP+ and WT mice from individual spines identified and counted under 100X magnification using stereological methods. Scale bar = 10 μm. E, F) A significant effect of genotype was found on spine density in the frontal cortex (E) (F(3,278) = 22.07, p < 0.0001), and hippocampus (F) (F(3,136) = 71.28, p < 0.0001). Spine density is expressed as number of spines per 10 μm dendrite length. Post-hoc testing showed significant decreases spine density in CT, TT, and AβPP+ mice as compared to WT mice (p < 0.01) in the frontal cortex and hippocampus, with TT mice having lower values as compared to CT and AβPP+ mice (p < 0.05) (*p < 0.05 compared to WT; #p < 0.05 compared to CT and AβPP+ mice). G) No effect of genotype was observed on spine density in the thalamus.

Article Snippet: At 6 months of age, mice were sacrificed and their brains were collected and subjected to Golgi staining (FD Rapid GolgiStain Kit; FD Neurotechnologies) according to the manufacturer’s instructions.

Techniques: Staining